BIOTECHNOLOGY

Paper 1

‘(THEORY)

(Three hours)

(Candidates are allowed  additional 15 minutes for only reading the paper. They must NOT start writing during this time.)

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Answer Questions I (compulsory)from Part I and five questions from Part Il.

The intended marks for questions or parts of questions are given in brackets [ ].

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PART I (Compulsory)

 

Question I

 (a)  Mention any one significant difference between each of the following: [5]

  1. Hybrid and cybrid.
  2. DNA polymerase and Taq DNA polymerase.
  3. Glycosidic bond and peptide bond.
  4. Oils and waxes.
  5. Homopolysacchartde and heteropolysaccharide.

 

(b)   Answer the following questions:[5]

  1. What Is a callus?
  2. Name the method used for the sterilization of plant hormones and vitamins.
  3. Why is DNA replication called semi-conservative replication?
  4. What is a promoter gene?
  5. State two uses of stem cells

(c) Write the full form of the following:[5]

(i) HGP                             (ii) STS                   {iii) CSIR                (iv) LAF                iv) SCP

(d)       Explain briefly.   [5]

(i) Amphipathic property of lipids.                                   (ii) Replication fork.

(iii) Androgenesis.               (iv) Transamination.                             (vi) Active site.

PART II

Answer any five questions

Question  [10]

(a)Explain the general structure of an amino acid. What do you understand by essential and non essential amino acids?

(b)What are cloning vectors? Write the main characteristics of any three types of cloning vectors.

(c) What is a codon? Name the start codon and any one end codon.

 

Question 3  [10]

(a)Briefly explain the structure of a tRNA molecule. Mention its function during the process of protein synthesis.

(b) Given the stepwise procedure of sequencing of DNA by Sanger’s method.

(c) What is totipotency?. Given an example of the totipotent cell.

 

Question 4  [10]

(a) Briefly describe the essential components of the nutrient medium used for the plant tissue culture technique. Also, write the names of any two plant tissue culture media frequently used in the laboratory.

(b)   With reference to suspension culture, explain the following:

.                                    (i) A chemostat.           (ii) A turbidostat.

(c)    What are purines and pyrimidines? Where are they located in a cell?

 

Question 5  [10]

(a)Explain giving an example how recombinant DNA technology can be used for the formation of the following:

.                                            (i) A vaccine.                                                                 (ii) A plant with delayed fruit ripening.

(b)   What is osmotic pressure? Explain any one biochemical technique based on osmotic pressure,

(c) What are dextro-rotatory and laevo-rotatory substances?

 

Question 6  [10]

(a) Write short notes on:

.                               (i) Single nucleotide polymorphism.                                   (ii) Bioinformatics databases.

(b)How are biomolecules separated by the following techniques:

.                           (i) Chromatography.                                                                       (ii) Centrifugation.

(c)Give two differences between enzymes and inorganic catalysts.

 

Question 7   [10]

(a)     Differentiate between:

  1. Prokaryotic genome and Eukaryotic genome.
  2.  Somatic embryo and zygotic embryo

(b) Explain how a genomic DNA library is formed. How does it differ from cDNA library?

(c)Name any two inborn metabolic disorders in human beings. Also, write one main symptom for each of them.

 

Question 8  [10]

(a) Give the step-wise procedure of Southern blotting technique. Mention any two  important applications of this technique.

(b) What are blunt ends and sticky ends? How are they formed?

(c) Name any two industrial enzymes and give their uses.

 

Question 9  [10]

(a) Enlist the main steps in the regeneration of a complete plant from an explain.

(b)   Given below is a list of four biomolecules found in a living cell. For each of them, write, the class of biomolecules they belong to and their location in a living cell:

(i) Histories.                                         (ii) Haemoglobin.
(iii) mRNA.                                         (iv) Glycogen.

(c) Write any two uses of transgenic plants.

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