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CBSE Class 12 Chemistry Notes: Biomolecules – Isoelectric Point

by Neepur Garg
April 22, 2019
in 12th Class, Class Notes
Reading Time: 3min read
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(i)   When ionized form of amino acid is placed in an electric field it will migrate towards the opposite electrode.

(ii)   Depending upon the pH of the medium following three thing may happen.

(a)  In acidic medium, the cation move towards cathode.

(b)  In basic medium, the anion move towards anode.

(c)  The Zwitter ion does not move towards any of the electrodes.

(iii)  At a certain pH (i.e. H+ concentration), the amino acid molecules show no tendency to migrate towards any of the electrodes and exists as a neutral dipolar ion, when placed in electric field is known as isoelectric point.

(iv)  All amino acids do not have the same isoelectric point & it depends upon the nature of  R – linked to α- carbon atom.

Illustration 1. Pick out incorrect statement

(A)  In an electrolysis experiment, α – amino acids do not towards any of the electrode at the isoelectric point.

(B)  p-aminobenzenesulphonic acid is a dipolar ion; while p-aminobenzoic acid does not

(C)  Sulphanilic acid is soluble in base, but not in acid

(D)  H3N+CH2COOH (pka = 2.4) is more acidic than RCH2COOH (pKa = 4 – 5)

Solution: (A) The pH at which |Anion| = |Cation| is called isoelectric point. At isolectric point, a – amino acids to not migrate when field is applied.

(B)  -SO3H is strongly acidic and donates H+ to weakly basic arylamino group. ArCOOH is not acidic enough to transfer H+ to the arylamino group.

(C)  In p- H3N+ C6H4SO3–, H3N+ is acidic enough to transfer H+ to bases to give the soluble anion p – H2N – C6H4SO3– – SO3 is too feebly basic and cannot accept H+ from acids.

(D)  α – H2N+ group increases acidity, because of its electron – withdrawing inductive effect.

Hence (D) is correct.

Isoelectric point of some amino acid 

Amino acid

Isoelectric point

Neutral amino acids

(pH 5.5 to 6.3)

Glycine

5.7

Alanine

6.1

Valine

6.0

Serine

5.7

Threonine

5.6

Acidic amino acids

(pH ≈ 3)

Aspartic acid

2.8

Glutamic acid

3.2

Basic amino acids

(pH ≈ 10)

Lysine

9.7

Arginine

10.8

(v)  Amino acids have minimum aqueous solubility at isoelectric point. 

 

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Neepur Garg

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